FIG 7.

(A) The γ₁34.5 protein forms a complex with UL31, UL34, PKC, and p32 in HSV-infected cells. Monolayer HeLa cells were mock infected or infected with HSV-1(F), H1001, or H1002 (5 PFU/cell). At 16 h postinfection, lysates of cells were immunoprecipitated with anti-γ₁34.5 antibody. Precipitated proteins and whole-cell lysates (WCL) were processed for immunoblotting with antibodies against PKCδ, p32, UL31, UL34, γ₁34.5, and tubulin. (B) UL31 and UL34 localize to the nuclear membrane independently of γ₁34.5. HeLa cells were mock infected or infected with the indicated viruses (5 PFU/cell). At 16 h postinfection, cells were stained with DAPI and antibodies against UL31 and UL34. Samples were then visualized by confocal microscopy.