FIG 8.

(A) The γ₁34.5 protein interacts with UL31. Monolayers of HeLa cells were transfected with γ₁34.5 along with a plasmid expressing UL31 or UL34. At 36 h after transfection, cells were processed for immunoprecipitation with anti-γ₁34.5 antibody. Precipitated proteins and whole-cell lysates were probed with antibodies against FLAG, γ₁34.5, and tubulin. (B) The amino terminus of γ₁34.5 interacts with UL31. HeLa cells were transfected with a vector plasmid or a plasmid expressing wild-type γ₁34.5 or the γ₁34.5 mutant devoid of amino acids 1 to 146 (DN) or amino acids 147 to 263 (DC). Cells were then processed for immunoprecipitation with anti-γ₁34.5 antibody. This was followed by Western blotting with antibodies against FLAG, γ₁34.5, and tubulin as for panel A.