Fig. 11.

The effect of JAS on P. falciparum intraerythrocytic development. (A,B) Pf lactate dehydrogenase (PfLDH) activity was measured in the ring (00-04 hours post-invasion; n=3), late ring (12–16 hours post-invasion; n=3), early trophozoite (20–24 hours post-invasion; n=3) and late trophozoite (28–32 hours post-invasion; n=3) stages. (B) PfLDH activity was measured following a 3-hour incubation with (white bars) and without (black bars; n=3) JAS. Data are normalized to number of parasites and are expressed as a percentage of PfLDH activity in untreated PE; error bars denote ±standard error. Statistical significance (asterisks) was determined using Student’s t-test, where P<0.05 when comparing untreated and JAS-treated PE within each intraerythrocytic stage. n, number of separate parasite cultures analyzed.