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Electron micrographs showing cytostome collar morphology in JAS-treated PE. (A–D) Single thin-section electron micrographs of trophozoite PE fixed with ‘mix-fix’ immediately following incubation with JAS. (A,B) The electron-dense ring is observed as two electron-dense collars (arrowheads). Note the radiating electron-dense pattern at the cytostome neck both in the horizontal view (C) and the top view (D). Scale bar: 100 nm.
