Fig. 4.

Limbal epithelial cell growth, colony forming efficiency and cell numbers in non-GMP and GMP-grade media. (A) Schematic graph showing the area of explant outgrowth (mm²) on different days of culture for the explants cultivated in non GMP (n = 4) and GMP-grade medium (n = 4); (B) The colony forming efficiency of cells from different zones of outgrowths cultivated in non- GMP (n = 4) or GMP-grade medium (n = 4) was performed as described in Section 2.5.; (C) Cell numbers per zone in non-GMP and GMP-grade media (n-4). Data are presented as mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001.