Figure 6.

RNAi knockdown of Tig or Tsp enhances fon-mediated muscle detachment. (A–H) Two hemisegments of the larval musculature stained for F-actin in a WT control, the indicated RNAi lines alone (B–D) or in a sensitized fon (fon^Δ24/+) genetic background (E–H). (B) RNAi knockdown of Tig results in missing muscles 6 and 7 (bracket) or muscles that lift off of the cuticle (indented arrowheads). (C and D) Loss of Tsp (C) mildly affects muscle attachment, while Gel (D) alone has no effect. Compared to the WT appearance of fon^Δ24/+, da-Gal4 alone (E), an enhancement of detached muscles (arrows) is observed upon concurrent expression of Tig RNAi (F) or Tsp RNAi (G), but not Gel RNAi (H). (I) Quantitation of muscle detachment in the indicated genotypes in a fon-sensitized background (14 ≤ n ≤ 29 for each genotype). The purple lines illustrate the predicted additive effects of each individual contribution. (J) qPCR results showing that the indicated RNAi lines effectively knockdown Tig, Tsp, and Gel transcripts. Mean ± SD; P-values: *** P < 0.005, ** P < 0.01, * P < 0.05; n.s. = not significant. Notations in purple indicate comparisons to fon^Δ24/+; da-Gal4 alone, P-values: ^#### P < 0.001, n.s. = not significant. Bars, 100 µm for A–G.