Mutations in MFP2, PED1, and CHY1 confer sucrose dependence and IBA and 2,4-DB resistance. (A–C) Chromosome map indicates the positions of markers used to map mfp2 (A) and chy1 (C) mutants and ratios of recombinants to the number of chromosomes assessed. Gene diagrams indicate the position of mfp2 (A), ped1 (B), and chy1 (C) mutations. MFP2 (A), PED1 (B), and CHY1 (C) were aligned with related proteins (Table S4); residues are shaded when identical (black or colors) or chemically similar (gray) in at least five (A) or four (B, C) sequences. (D) Hypocotyl lengths of 5-day-old dark-grown seedlings were measured. Error bars show SD (n = 20). (E) Root lengths of 8-day-old light-grown seedlings were measured. Error bars show SD (n ≥ 15). Data are representative of three replicates (D, E).