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(A) Molecular model of ArgO. The first 38 residues comprising the cytoplasmic NTD are not included in this model. The locations of the cysteine substitutions that formed the basis for the topological assignment are indicated. On the basis of experimental observations, a monomer model of ArgO was designed. (B) The positions of two amino acid substitutions that disable ArgO function (red) and their compensatory amino acid substitutions (green) in the ArgO model are shown.
