Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Kinetic constants for the flavokinase/FAD synthetase reactions of Lmo0728 (27.7 kDa) and Lmo1329 (35.5 kDa) from Listeria monocytogenes
| Enzymea | Substrate | Km (μM) | Vmax (U mg−1)b | kcat (s−1) | kcat/Km (μM−1 s−1) |
|---|---|---|---|---|---|
| Lmo1329 (FK) | Riboflavin | 6.9 | 668 | 0.40 | 0.057 |
| Lmo1329 (FS) | FMN | 29.2 | 3,706 | 4.39 | 0.150 |
| Lmo0728 (FS) | FMN | 12.9 | 1,093 | 0.51 | 0.040 |
FK, flavokinase; FS, FAD synthase.
Specific activities (U mg−1) are in nmol min−1 mg−1 protein.
