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. 2016 Jun 21;15(21):2882–2894. doi: 10.1080/15384101.2016.1198861

Figure 5.

Figure 5.

Retroviral expression of Cyclin D3 in pre-B cells prevents repression of Cyclin D3 Protein and impairs G1 arrest in response to DSBs. (A) Representative Western blot analyses of Cyclin D3 and actin protein in unirradiated or irradiated IL7-cultured EμBCL2:Ccnd3−/− pre-B cells transduced with MIGR1 or MIGR1-D3 at indicated times following exposure to 10 Gy of IR. (B) Representative flow cytometry analysis of the cell cycle of unirradiated or irradiated IL7-cultured EμBCL2 pre-B cells transduced with MIGR1 or MIGR1-D3 at 6 hours after exposure to 10 Gy of IR. (C) Graph depicting quantification of the fraction of unirradiated or irradiated IL7-cultured EμBCL2 pre-B cells transduced with MIGR1 or MIGR1-D3 that are BrdU+ at 6 hours following IR. This experiment was conducted 3 times. Error bars are standard error. *p < 0.05. (D) Graph depicting the ratios of the fraction of each cell type in B and C that synthesizes DNA after IR vs. before IR. Error bars are standard error. *p < 0.05.