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. 2016 Aug 25;15(21):2920–2930. doi: 10.1080/15384101.2016.1224043

Figure 6.

Figure 6.

Ectopic expression of exogenous p21 substantially reversed the effect of miR-33b-3p on cisplatin resistance. (A) Over-expression of p21 in A549 cells. Western blot was used to monitor the expression level of p21 in A549 cells 48h after transfection with pcDNA3.0-p21△3′UTR (also designated as p21), pcDNA3.0-p21 with 3′UTR or empty pcDNA3.0 vector, GAPDH was used as an internal control. (B) and (C) Expression of exogenous p21 protein significantly abrogated the enhanced proliferation conferred by miR-33b-3p under cisplatin treatment. Columns, mean of at least 3 independent experiments; bars, SEM. *, P < 0.05; comparison between 2 groups as indicated. (D) Cell cycle analysis of of cells treated as (B). The percentage of G1, S, and G2/M are demonstrated as shown.