Figure 3.
Analysis of expression of genes involved in regulation of cell cycle in both Pax7ko and Pax7wt ESCs and MEFs. (A). Gene Ontology analysis of significantly expressed genes in differentiating ESCs and asynchronously proliferating MEFs showing proportion of cell cycle related processes among all others. List of the processes is presented in Tables S1 and S2; (B). Microarray analysis of the expression levels of transcripts that encoded cell cycle-associated proteins in undifferentiated ESCs and in EBs at day 7 and EBOs at day 21 of differentiation (left diagram) and asynchronously proliferating MEFs (right diagram). For each cell type and genotype one cell line was analyzed. Each group included triplicate measurements. Blue color indicates low expression and red color indicates high expression levels of transcripts. Genes which levels change similarly in differentiating ESCs and MEFs were marked in green. All genes shown were selected from the database complete list presented in Czerwinska et al.5 and Table S3.