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. 2016 Sep 9;15(21):2931–2942. doi: 10.1080/15384101.2016.1231260

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© 2016 The Author(s). Published with license by Taylor & Francis

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 7. — Analysis of apoptosis rate of both Pax7ko and Pax7wt ESCs and MEFs. (A). The proportion of cells expressing γ-H2A.X in EB outgrowths at 14 day of differentiation. P-value: ***<0.001. (B). Localization of γ-H2A.X (green) and nuclei (red) in EB outgrowths at 14 day of in vitro culture; bar 50 µm. (C). The proportion of cells expressing γ-H2A.X in quiescent (left diagram) and cycling (right diagram) MEFs. (D). Localization of γ-H2A.X (green) and nuclei (red) in cycling MEFs; bar 50 µm. For each cell type and genotype 3 cell lines were analyzed, graphs represent the median values.