Fig 5. GLS1 silencing enhances DHA-induced cytotoxicity in LM3 cells.
a, The expression of GLS1 protein was decreased after cells were transfected with GLS1-siRNA for 48 h. Blots are representative of three independent experiments. b, The combinatory cytotoxicity of GLS-siRNA and DHA was assessed by the MTT assay after LM3 cells were treated with GLS-siRNA and/or DHA (20 μM) for 48 h. Means ± SD are shown. Similar results were obtained in three independent experiments. * P < 0.05, *** P < 0.001.