Fig 4. RhLIGHT enhances the viability and proliferation of human BM-MSCs.

Cells were incubated with 0, 100, and 200 ng/mL rhLIGHT for 72 h. (A) Cell viability of BM-MSCs, as determined by MTS assay. (B) Expression of survival proteins and anti-apoptotic proteins, as determined by western blotting. (C) Cell proliferation of BM-MSCs, as determined by BrdU assay. (D) Cell cycle distribution of BM-MSCs, as determined by PI/RNase assay (E) Expression of cell cycle-related proteins, as determined by western blotting. The membrane was stripped and reprobed with anti-β-actin mAb to confirm equal loading. Data represent the mean ± SEM. Significantly different from the control cells (*); ***, P < 0.001.