Table 1. Antibodies and processing used in immunohistochemical analyses.
| Antibody | Origin | Pre-treatment | Specie | Clone | Dilution | Secondary Antibody |
|---|---|---|---|---|---|---|
| Aquaporin 1 | Sigma (St. Louis, MO/USA) | Citrate | Rabbit | Polyclonal | 1:200 | Envision (Dako—Glostrup, Denmark) |
| Aquaporin 3 | Abcam (Cambridge, MA/USA) | Citrate | Rabbit | Polyclonal | 1:1500 | Novolink (Leica—Wetzlar, Germany) |
| Aquaporin 5 | Abcam (Cambridge, MA/USA) | Citrate | Rabbit | EPR3747 | 1:400 | Novolink (Leica—Wetzlar, Germany) |
| Na-K-ATPase(α) | Abcam (Cambridge, MA/USA) | Citrate | Mouse | 464.6 | 1:800 | Novolink (Leica—Wetzlar, Germany) |
| ENaC(δ) | Abcam (Cambridge, MA/USA) | Citrate | Rabbit | Polyclonal | 1:2500 | Envision (Dako—Glostrup, Denmark) |
ENaC: Epithelial Na+ Channel