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. 2016 Nov 11;2(11):e1600990. doi: 10.1126/sciadv.1600990

Fig. 4. Assessment of cold and heat sensitivity in cultured DRG neurons in vitro.

Fig. 4

(A) In vitro images showing changes in GCaMP3 fluorescence from cultured DRG neurons at rest (basal) and in response to perfused 0°C extracellular solution, 55°C extracellular solution, and KCl. Unimodal (white arrowheads) and polymodal (yellow arrowheads) neurons are shown. Scale bars, 10 μm. (B) Heatmap response profiles for every neuron responsive to 0° or 55°C stimuli. Each row displays the maximum normalized fluorescent signal acquired during each application window (n = 171).