Table 1. Primers for PCR and sequencing.
| Set | Direction | Primer Name | Primer Sequence | Binding Site (from EcoRI site) | Fragment Length | Number of Samples |
|---|---|---|---|---|---|---|
| PCR Primers | ||||||
| A | Forward | CoreF | 5′-GTGTGGATTCGCACTCCT-3′ | 2269-2287 | 2.1 kb | 65 |
| Reverse | Werle AS | 5′-CGTCAGCAAACACTTGGC-3′ | 1175-1192 | |||
| B | Forward | P6 | 5′-GGGCAGGTCCCCTAGAAGA AGAACT-3′ | 2363-2386 | 2.2 kb | 10 |
| Reverse | FengR | 5′-CCGATGAGCTTTGCTCCAGACC-3′ | 1328-1307 | |||
|
| ||||||
| Sequencing Primers | ||||||
| Reverse | Werle AS | 5′-CGTCAGCAAACACTTGGC-3′ | 1175-1192 | |||
| Forward | P7 | 5′-GTGGGTCACCATATTCTTGGG-3′ | 2820-2840 | |||
| Forward | P9 | 5′-ATTCCTATGGGAGTGGGCCTCAG-3′ | 633-655 | |||
| Reverse | P15 | 5′-ATAACTGAGAGCCAAACAGTGGG-3′ | 718-740 | |||