Fig. 2. qRT-PCR for selected target genes in the CT group compared with sham and CI groups. The mRNA levels of Ephb1, Zfp90, Prdx6, Spg7, and Clic4 were measured by qRT-PCR and calculated by the ΔΔCt methodology as described in methods. The assays were run in triplicate, and the standard error of the mean (SEM) was determined. Relative expression of Zfp90, Prdx6, and Spg7 were markedly decreased in the CT group compared with the CI group, but changes in Ephb1 did not reach statistical significance (A-D). mRNA for Clic4 was markedly decreased in the CI group but was rescued in the CT group (E; n=5 per group). Data are presented as mean±SEM. ^*p<0.05, ^**p<0.01 vs. CT group. qRT-PCR : quantitative real-time PCR, CI : cerebral ischemia, mRNA : messenger ribonucleic acid, EPHB1 : eph receptor B1, ZFP90 : zinc finger protein 90, PRDX6 : peroxiredoxin 6, SPG7 : paraplegin matrix AAA peptidase subunit, CLIC4 : chloride intracellular channel 4, GAPDH : glyceraldehyde 3-phosphate dehydrogenase.