Fig. 1. Expression of TonEBP during adipocyte differentiation.

3T3-L1 preadipocytes were induced to differentiate into adipocytes. (A) Total cell lysates were prepared at indicated times and protein expression was analyzed by immunoblotting. (B) TonEBP expression (A) was normalized to that of GAPDH and then presented as a relative value based on D0. (C) Expression of TonEBP mRNA at indicated times was measured by quantitative RT-PCR and presented as a relative value to D0. GAPDH mRNA expression was used as a reference. Values are mean±SEM. ^*p<0.05, ^**p<0.01 compared with D0 for each group (n≥3).