Fig. 2. Suppression of adipocyte differentiation by TonEBP.

3T3-L1 cells were infected with recombinant adenovirus expressing β-galactosidase or TonEBP and then induced to differentiate into adipocytes. Expression of TonEBP (A) or adipogenic proteins (D) was analyzed by immunoblotting. (B) The degree of adipocyte differentiation was visualized by staining lipid droplets with Oil Red O at D6. (C) Lipid droplets shown in (B) were extracted with isopropyl alcohol and quantified as a relative value to Adβ-gal. (E) The quantity of FABP4 shown in (D) was normalized to that of GAPDH and then presented as a relative value to D6 of Adβ-gal. Values are mean±SEM. ^*p<0.05, ^**p<0.01 compared to Adβ-gal for each group (n=3).