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. 2016 Oct 28;20(6):649–655. doi: 10.4196/kjpp.2016.20.6.649

Fig. 3. Promotion of adipocyte differentiation by TonEBP knockdown.

Fig. 3

3T3-L1 cells were transfected with scrambled or TonEBP siRNA and then induced to differentiate into adipocytes. Expression of TonEBP (A) or adipogenic proteins (D) were analyzed by immunoblotting. (B) The degree of adipocyte differentiation was visualized by staining lipid droplets with Oil Red O at D6. (C) Lipid droplets shown in (B) were extracted with isopropyl alcohol and quantified as a relative value to scrambled siRNA. (E) The quantity of FABP4 shown in (D) was normalized to that of GAPDH and then presented as a relative value to D6 of scrambled siRNA. Values are mean±SEM. *p<0.05, **p<0.01 compared to scrambled siRNA for each group (n≥3).