Fig. 4. Limited anti-adipogenic property of TonEBP in the early stages of adipocyte differentiation.

3T3-L1 cells were treated with differentiation factors at D0 for adipogenesis. Recombinant adenoviruses were infected at indicated times as described in (A). (B) Expression of each protein was analyzed by immunoblotting. (C) The degree of adipocyte differentiation was visualized by staining lipid droplets with Oil Red O at D6. (D) The quantity of FABP4 shown in (B) was normalized to that of GAPDH and then presented as a relative value to D-3 of Adβ-gal. Values are mean±SEM. ^**p<0.01, ^***p<0.001 compared to Adβ-gal for each group (n=6).