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. 2016 Oct 28;20(6):649–655. doi: 10.4196/kjpp.2016.20.6.649

Fig. 5. Suppression of MCE and insulin signaling with TonEBP overexpression.

Fig. 5

3T3-L1 cells infected with recombinant adenoviruses were treated with differentiation factors (IDI) for the indicated times. (A) Cell number was determined via MTT assay and presented as a relative value to DO. (B) The protein levels of phosphorylated IRS-1 (p-IRS-1), IRS-1, phosphorylated Akt (p-Akt), and Akt in these cells were detected by immunoblotting using specific antibodies. The experiment was repeated three times with similar results.