Figure 5.

Chromatin Landscapes Are Immediately Modified after Severe Muscle Trauma and Reflect Recruitment of Different Types of Immune Cells

(A) Heatmap of p values for over-represented pathways derived from enriched H3K27ac peaks in the early time period (3–48 hr). AP-1, activator protein 1; CXCR2, C-X-C motif chemokine receptor 2; FC gamma R, Fc-γ receptor; GM-CSF, granulocyte macrophage colony-stimulating factor; HIF-1α, hypoxia-inducible factor 1α; IL8, interleukin-8; NF-κβ, nuclear factor κB; TNF, tumor necrosis factor.

(B) Bar graphs of individual gene expression values through time from left to right of immune-cell related transcripts upregulated in the early period (injured samples are colored and uninjured samples are uncolored). Error bars represent 1 SD.

(C) Normalized ChIP-seq tracks of H3K4me1, H3K27ac, and H3K4me3 profiles around the Atf3 gene. Enriched enhancer regions are highlighted in gray.

(D) Heatmap of over-represented pathways derived from enriched H3K27ac peaks in the middle time period (48–336 hr). GPCR, G-protein-coupled receptor; IL-4, interleukin-4; MMP, matrix metalloprotein.

(E) Histograms of individual gene expression values through time from left to right of anti-inflammatory related transcripts. Interleukin-4 receptor α (Il4ra), interleukin-10 receptor α (Il10ra), and interleukin-13 receptor α 1 (Il13ra1), colony-stimulating factor 1 receptor (Csf1r), and peroxisome proliferator-activated receptor γ (Pparg). Error bars represent 1 SD.

(F) Normalized ChIP-seq tracks of H3K4me1, H3K27ac, and H3K4me3 profiles around the Ppar-γ gene. Enriched enhancer regions are highlighted in gray.