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. 2016 Oct 20;7(5):927–940. doi: 10.1016/j.stemcr.2016.09.011

Figure 3.

Figure 3

Analysis of Methylation Levels in the Region Upstream of the G4C2 Repeats and in the G4C2 Repeat Itself in Primary Fibroblasts and iPSCs Derived from Patient M

(A) Methylation levels upstream of the repeats were determined by bisulfite sequencing of the same region as in Figure 1A in WT and C9 affected parental fibroblasts derived from a 65-year-old C9/ALS-manifesting patient (patient M) and iPSCs derived from them (C9 iPS#M1, iPS#M9, and iPS#H10). Each line represents the upstream region of the repeats, with methylated and unmethylated CpGs designated by black and white circles, respectively.

(B) Results of the (G4C2)n-methylation assay in primary fibroblasts and iPSC clones derived from patient M. Left panel (blue channel) represents methylated alleles while the right panel (green channel) represents unmethylated alleles.