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. 2016 Oct 27;7(5):911–926. doi: 10.1016/j.stemcr.2016.09.012

Figure 1.

Figure 1

Generation of Dual Transgenic Mice (O4-DE-GFP/O4-PE-RFP) and the Distinct Oct4 Regulatory Elements in the Totipotent Cycle

(A) Physical maps of wild-type endogenous Oct4, Oct4-ΔPE-GFP (O4-DE-GFP), and Oct4-ΔDE-RFP (O4-PE-RFP).

(B) The expression pattern of O4-DE-GFP and O4-PE-RFP in pre-implantation embryos (2C, blastocyst). The O4-DE-GFP/O4-PE-RFP pre-implantation embryos expressed only O4-DE-GFP. Scale bars, 50 μm.

(C–H) The expression pattern of O4-DE-GFP and O4-PE-RFP in post-implantation embryos. Phase and fluorescence images of (C) 6.5-dpc embryo (scale bar, 100 μm); (D) 7.25-dpc embryo (scale bar, 100 μm); (E) 8.5-dpc embryo (scale bar, 100 μm); (F) 9.5-dpc embryo and migrating PGCs (scale bar, 100 μm); (G) 10.5-dpc embryo and PGCs (scale bar, 100 μm); and (H) 13.5-dpc embryo and gonad (scale bar, 100 μm). Arrowheads indicate GFP-positive areas in the developing gonads.

(I and J) The expression pattern of O4-DE-GFP and O4-PE-RFP in testis. Phase and fluorescence image of (I) 7-dpp testis and seminiferous tubules and (J) 4-week-old adult mouse testis and seminiferous tubules. Scale bars, 100 μm.

(K) The neurosphere did not express either O4-DE-GFP or O4-PE-RFP. Scale bar, 100 μm.