Figure 6.

Epigenetic Status of Oct4 Regulatory Elements in Naive and Primed Pluripotent Stem Cells

(A) Analysis of Oct4 enhancer activity in ESCs, 2i-GFP⁺ cells, GFP⁺ GR⁺ cells, RFP⁺ cells, EpiSCs, and P19 embryonic carcinoma cells. Relative luciferase activity was normalized to the activity of the empty vector. Data are presented as mean ± SEM for n = 3 independent experiments.

(B) ChIP-qPCR analysis to determine immunoglobulin G (IgG), H3K27ac, H3K27me3, and H3K9me3 enrichment on the Oct4 distal and proximal enhancers. Data are presented as mean ± SEM for n = 3 independent experiments. Coloured stars indicate histone tails, such as H3K9 and H3K27.

(C) ChIP-qPCR analysis to determine IgG and Nanog enrichment on the Oct4 distal and proximal enhancers. Data are presented as mean ± SEM for n = 3 independent experiments.

(D) Bisulfite genomic sequencing of the regions of the Oct4 DE, PE, and PP in 2i-GFP⁺, GFP⁺, GR⁺, and RFP⁺ cells, and MEF. Black and white circles represent methylated and unmethylated CpGs, respectively.