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. 2016 Nov 11;6:57. doi: 10.1186/s13578-016-0122-6

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Characterization of retroviral vector for one-step immortalization. a Schematics of the MSCV-pic2 vector design. For one-step immortalization, a hTERT cDNA and a shRNA targeting p16^INK4A and p14^ARF were introduced into this vector. b Western blot verifying the knockdown of p16^INK4A and p14^ARF in stably transduced SAECs. c Telomerase activities in SAECs stably transduced with hTERT cDNA (error bars represent SD for all bar charts unless otherwise stated)