Table 4.
Inter-assay variability of internal control
| Plates | IL-17F | GM-CSF | IFNγ | IL-10 | CCL20/MIP3α | IL-13 | IL-17A | IL-22 | IL-9 | IL-2 | IL-5 | IL-27 | TNFα |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 2091.2 | 7423.2 | 5300.1 | 81.4 | 9523.6 | 2248.3 | 74.0 | 45.4 | 225.6 | 89.8 | 191.9 | 808.9 | 3424.9 |
| 2 | 2059.3 | 6734.8 | 6833.8 | 82.0 | 9009.3 | 2537.1 | 90.9 | 65.0 | 256.9 | 107.0 | 224.2 | 1081.6 | 4126.7 |
| 3 | 1963.3 | 6811.0 | 5144.7 | 74.7 | 5706.4 | 2268.3 | 82.2 | 46.3 | 216.1 | 90.9 | 214.7 | 786.4 | 3571.6 |
| 4 | 2215.3 | 8326.4 | 5348.7 | 78.4 | 8241.1 | 2412.0 | 83.1 | 53.8 | 231.1 | 104.7 | 205.1 | 1308.3 | 3953.4 |
| 5 | 1998.1 | 6735.9 | 5506.4 | 61.1 | 8311.7 | 2064.6 | 72.1 | 48.5 | 195.9 | 89.2 | 172.9 | 1026.8 | 3385.1 |
| 6 | 2238.5 | 7407.3 | 6040.5 | 85.1 | 6113.7 | 2259.9 | 89.1 | 61.5 | 263.3 | 84.2 | 218.3 | 1093.6 | 4302.2 |
| 7 | 2473.7 | 8788.0 | 5809.2 | 71.3 | 8211.6 | 2560.0 | 88.3 | 47.0 | 278.8 | 98.5 | 243.6 | 1102.3 | 4128.4 |
| 8 | 2425.1 | 7440.4 | 5180.8 | 81.6 | 10147.7 | 2429.5 | 84.7 | 50.8 | 254.4 | 105.9 | 214.3 | 1190.2 | 4310.1 |
| 9 | 1945.9 | 6719.6 | 4545.8 | 68.4 | 8901.7 | 2384.8 | 82.4 | 53.3 | 198.0 | 95.8 | 221.2 | 1175.7 | 4241.0 |
| 10 | 2165.8 | 7817.6 | 7062.7 | 88.5 | 10767.6 | 2729.8 | 87.8 | 50.3 | 296.4 | 110.3 | 241.2 | 1070.5 | 5285.1 |
| 11 | 2185.4 | 6660.9 | 4907.7 | 68.6 | 8756.7 | 2387.7 | 70.6 | 39.6 | 180.1 | 91.9 | 170.5 | 914.5 | 3409.2 |
| 12 | 2151.4 | 7223.0 | 5796.1 | 65.0 | 8429.9 | 2304.6 | 71.1 | 42.6 | 224.8 | 86.8 | 195.3 | 1088.9 | 4515.4 |
| 13 | 2231.2 | 7964.7 | 5324.4 | 78.5 | 7028.9 | 2618.0 | 87.1 | 51.7 | 237.8 | 113.9 | 230.2 | 1249.2 | 4951.5 |
| %CV | 7.4 | 9.2 | 12.8 | 11.0 | 17.2 | 7.4 | 9.0 | 14.0 | 14.4 | 10.0 | 11.0 | 14.6 | 14.3 |
Mean concentration values of duplicate Internal Controls (pg/ml) and %CVs of mean concentration calculated using the 13 inter-plates internal control mean concentration values for the selected 15-plex Millipore magnetic kit (see Material and Methods section for detailed analytes). IL-23 and IL-1β %CVs were not calculated due to concentration values < LLOQ and > ULOQ, respectively