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. 2016 Nov 11;14:312. doi: 10.1186/s12967-016-1071-x

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Outcomes of cells cultured with BAL fluid from mice with ARDS. a Proliferation of splenocytes from naïve mice cultured with BAL fluid from mice with ARDS (BAL, n = 5, experiments in triplicate) compared to cells cultured with BAL fluid where the sPD-1 was precipitated out (Anti-sPD-1, n = 5, experiments in triplicate) Analysis done by t test, *p = 0.032 when compared to cells cultured with BAL fluid where sPD-1 was precipitated out. b TNF-α levels of splenocytes from naïve mice cultured with BAL fluid from mice with ARDS (BAL, n = 5) compared to cells cultured with BAL fluid where the sPD-1 was precipitated out (Anti-sPD-1, n = 5) Analysis done by t test, *p = 0.002 when compared to cells cultured with BAL fluid from mice with ARDS. c IL-6 levels of splenocytes from naïve mice cultured with BAL fluid from mice with ARDS (BAL, n = 5) compared to cells cultured with BAL fluid where the sPD-1 was precipitated out (Anti-sPD-1, n = 5)