Table 5.
PCR primers, product size and their physical position designed from whole-genome shotgun strategy
| Marker name | Forward sequence | Reverse sequence | Size | POPSEQ Phyiscal Position |
|---|---|---|---|---|
| InDel-1 | TTATCCCCACTTACAGCCCG | GGTAGTACCGCTAGGGGCA | 80 bp | Chr 5H 487,228,854 |
| InDel-2 | GTTTAGCAGGGAGGCTCGAA | GAATATCTGGCCATGCATGCA | 130 bp | Chr 5H 482,479,881 |
| InDel-3 | GCGAAACCTTCCTTGCTTCC | TTTGACAAGGTTGGTCTCAAAAA | 125 bp | Chr 5H 481,610,815 |
| InDel-4 | AGCCAACGGTAGGTACATGC | GCTGCTGCTAGGTCAGAAAGA | 122 bp | Chr 5H 487,890,216 |
| InDel-5 | CACCCATATTTGTGCATAGGC | TGAGGTTGTGGATTGACGAACA | 120 bp | Chr 5H 486,795,826 |
| InDel-6 | TCAGGTCCCTTCTTCAGTGA | AGTGCCAGCCAAATGCAGTA | 106 bp | Chr 5H 482,690,738 |
| InDel-7 | GTTTAAAGCCGGTGAGCGTG | ACGTTGTTATTTATACAACACAGGG | 83 bp | Chr 5H 482,623,538 |
| InDel-8 | AGGAATTAACAGGACAAATTTAGCA | TCATGGTGAAACATGGTGAATCT | 107 bp | Chr 5H 481,623,681 |
| InDel-9 | GGTACTACCGCTCGCGAG | GCCAGCAGTAGTACCGCTAG | 101 bp | Chr 5H 481,632,966 |
| InDel-10 | CAATTGTTCAGGCAAAAATTCA | CTCTCGAGGAAAGCAGGTAATG | 139 bp | Chr 5H 481,342,502 |
| InDel-11 | GATACTGAAATCTTGCCCATGC | TCAATTGCTTTGTTTGTGGAAC | 180 bp | Chr 5H 481,701,251 |
| InDel-12 | TGTTGCCCTCTGGTATGAATAG | AATTTCAGTGTCTGAACTATGGG | 136 bp | Chr 5H 482,525,089 |
| InDel-13 | CCTTCTTCGTCTAGCACCCATA | TGCTCGTACTGAGGTTTCTTGA | 175 bp | Chr 5H 486,795,676 |
| InDel-14 | ACAGTAGCGCCAGTAATTGTGT | AGTTTCCAGAGTATCACTGCCA | 180 bp | Chr 5H 487,123,960 |
| InDel-15 | TGTGTGGCAGTTTCTTTAATGG | TGAAGCTTTGTTTTACTGACGG | 176 bp | Chr 5H 487,521,136 |
| InDel-16 | AACAAGACCTGGAGAGACAAGC | TGCTGCTAGGTCAGAAAGAAAA | 138 bp | Chr 5H 487,890,066 |
| InDel-17 | CTCCTACCACCCTTTTCACCC | TACTGCAAGAAATCGTACCACC | 154 bp | Chr 5H 488,465,184 |