Fig. 1.

Co-immunoprecipitation of KMT2A and Msk1. a Western blots analysing KMT2A-immunoprecipitated material from human lymphoblastoid cells (LCLs), mouse embryonic fibroblasts (MEFs) and human embryonic kidney cells (HEKs). The precipitated material tested positively for Msk1. A representative western (1 out of 3) is shown. b Western blots analysing Msk1-immunoprecipitated material from the indicated cell lines. The precipitated material tested positively for KMT2A. A representative western (1 out of 3) is shown. c FLAG-KMT2A was overexpressed in HEK293 cells, immunoprecipitated using FLAG antibodies and characterised by SDS gel (silver nitrate stain, left panel). The gel was Western-blotted and the filter-stained sequentially with antibodies to FLAG, phosphorylated Msk1 and NFκB as indicated