Cytochrome oxidase subunit I (COI) Maximum Parsimony tree, one of the two most parsimonious trees that did not differ in any arrangements after collapse of all branches with <50% bootstrap support (1,000 replicates). Circle—UV trap outdoors, Square—CDC standard light trap indoors, Filled Circle—CSP-ELISA positive, An. gambiae from barrier collection in northern Zambia, An. coluzzi from insectary at Johns Hopkins Bloomberg School of Public Health, +—CSP-ELISA positive, *—P. falciparum qPCR positive.