Table 1.
Primer pairs of DNA sequences for used proteins (or polypeptide fragments) and their expected molecular weight including HisTag
| Protein | Forward primer 5′-3′ | Reverse primer 5′-3′ | Expected protein molecular weight |
|---|---|---|---|
| BamB | ATGCAATTGCGTAAATTACTTCTGCC | TTAACGCGTAATCGCGTAGACC | 45.8 kDa |
| FliC-frag | ACGCTGAATGTGCAACAAAAATATAAGGTC | ACCTTCGGCTTTACTTGCAGCG | 25.8 kDa |
| MetQ | ATGGCGTTCAAATTCAAAACCTTTGCGG | TTACCAGCCTTTCACCGCGCC | 33.3 kDa |
| PrgI | ATGGCAACACCTTGGTCAGGCTATC | TTAACGGAAGTTCTGAATAATGGCAGCATC | 12.7 kDa |
| SipC | ATGTTAATTAGTAATGTGGGAATAAATCCCGCC | TTAAGCGCGAATATTGCCTGCGAT | 46.8 kDa |
| SipD | ATGCTTAATATTCAAAATTATTCCGCT | TTATCCTTGCAGGAAGCTTTT | 41.0 kDa |
| SseB | ATGTCTTCAGGAAACATCTTATGGGGAA | TCATGAGTACGTTTTCTGCGCTATC | 25.4 kDa |
| SurA | ATGAAGAACTGGAAAACGCTGCTTC | TTAGTTACTCAAAATCTTAACGTAAGCGCTG | 51.1 kDa |
| SipB | ATGGTAAATGACGCAAGTAGCATTAGCCG | TTATGCGCGACTCTGGCGCAGAATAAAAC | 66.3 kDa |
| OppA | ATGTCTAACATCACGAAAAAAAGTTTG | TTAATGTTTGATAATATATAAGTTTTTCACATAAAT | 65.1 kDa |