Extended Data Table 1. Phenotypic analysis of the hematopoietic system in Mfn2^fl/fl-Vav-Cre mice.

Analysis of 8-10 week-old Mfn2^fl/fl or Mfn2^fl/fl-Vav-Cre mice for (A) frequency of LSK cells and HSCs, (B) peripheral blood counts, (C) BM progenitor populations (MPPs (lin⁻Sca1⁺kit⁺CD48⁺), CMPs (lin⁻Sca1⁻kit⁺), CLPs (lin⁻Sca1^lokit^loIL7Ra⁺Flt3⁺), MEPs (Lin⁻Kit⁺Sca1⁻CD16/32^lowCD34⁻)), (D) myeloid (Gr1⁺/Mac1⁺), T (CD3⁺) and B (CD19⁺) cells in the spleen, (E) myeloid (Gr1⁺/Mac1⁺), T (CD3⁺) and B (CD19⁺) cells in peripheral blood mononuclear cells, and (F) thymocyte subpopulations (DP: CD4⁺CD8⁺, DN: CD4⁻CD8⁻, DN1: CD4⁻CD8⁻CD44⁺CD25⁻, DN2: CD4⁻CD8⁻CD44⁺CD25⁺, DN3: CD4⁻CD8⁻CD44⁻CD25⁺, DN4: CD4⁻CD8⁻CD44⁻CD25⁻) Tables, mean±s.e.m.; n=3 mice; *P<0.05; two-tailed student’s t test.

A
Hematopoietic Stem Cell Compartments
	LSK	LSK CD48- Flt3- CD150+
Mfn2 f/f
	0.277%	0.053%
	0.319%	0.082%
	0.346%	0.058%
Mean	0.314% ± 0.03	0.064% ± 0.02*
Mfn2 f/fVav-Cre
	0.213%	0.021%
	0.575%	0.047%
	0.220%	0.039%
Mean	0.336% ± 0.20	0.036% ± 0.01*

B
Peripheral blood counts
	WT	KO
N=	8	4
WBC	7.3 ± 2.6	8.7 ± 1.7
Neutrophil%	15.4 ± 9.2	15.4 ± 12.0
Lymphocytes%	73.0 ± 12.8	78.2 ± 12.6
Monocytes%	6.2 ± 2.8	5.9 ± 3.2
Eosinophiles%	0.4 ± 0.3	1.3 ± 1.4
Basophiles%	0.1 ± 0.1	0.4 ± 0.4
NRBC%	±	±
Hematocrit	45.1 ± 4.2	45.3 ± 6.7
RBC	8.7 ± 0.7	8.9 ± 1.2
HB	12.3 ± 1.2	12.8 ± 1.6
MCV	51.5 ± 1.7	50.7 ± 1.8
MCH	14.1 ± 0.5	14.4 ± 0.5
MCHC	27.3 ± 0.8	28.4 ± 0.7
RDW	15.2 ± 1.7	15.5 ± 1.4
RSD	7.9 ± 0.9	7.9 ± 0.9
RETIC#	47.4 ± 29.9	49.1 ± 42.0
RETIC%	0.6 ± 0.4	0.6 ± 0.5
Platelet	642.8 ± 146.4	653.0 ± 66.7

C
Bone Marrow Progenitor Compartments
	MPP	GMP	CMP	MEP	CLP
Mfn2 f/f
		0.00272%	0.00184%	0.00281%	0.00049%
		0.00841%	0.00469%	0.00958%	0.00067%
		0.00149%	0.00164%	0.00135%	0.00409%
Mean	0.314% ± 0.03	0.0042% ± 4e-5	0.0027% ± 2e-5	0.0046% ± 4e-5	0.00175% ± 2e-5
Mfn2 f/f Vav-Cre
		0.00321%	0.00224%	0.00369%	0.00108%
		0.00844%	0.00550%	0.00589%	0.00041%
		0.00136%	0.00110%	0.00142%	0.00916%
Mean	0.336% ± 0.20	0.0043% ± 4e-5	0.0029% ± 2e-5	0.0037% ± 2e-5	0.00355% ± 5e-5

D
Splenocyte Lineages
	Mac + or GR1 +	CD3+	CD19+
Mfn2 f/f
	6.840%	17.60000%	59.70000%
	9.910%	27.80000%	63.70000%
	8.375%	22.700%	61.700%
Mean	8.37% ± 0.02	22.70% ± 0.05	61.70% ± 0.02
Mfn2 f/f Vav-Cre
	11.800%	13.79000%	62.59000%
	9.070%	27.50000%	63.20000%
	10.435%	20.645%	62.895%
Mean	10.43% ± 0.01	20.65% ± 0.07	62.90% ± 0.01

E
Peripheral Blood Lineages
	Mac1+ or GR1 +	CD3+	CD19+
Mfn2 f/f
	26.300%	9.75280%	54.75000%
	23.100%	26.800%	42.135%
	19.900%	43.84800%	29.51910%
Mean	23.1% ± 0.03	26.8% ± 17	42.1% ± 13
Mfn2 f/f Vav-Cre
	15.200%	15.63765%	63.22500%
	17.900%	35.944%	41.143%
	20.600%	56.24940%	19.06100%
Mean	17.9% ± 0.02	35.9% ± 20	62.9% ± 22

F
Thymus Lineages
	CD4	CD8	DP	DN	DN1	DN2	DN3	DN4
Mfn2 f/f
	10.50%	2.95%	81.60%	2.11%	0.03%	0.10%	0.54%	0.19%
	11.10%	2.10%	81.30%	3.10%	0.04%	0.15%	0.81%	0.17%
	5.95%	3.01%	83.20%	1.48%	0.03%	0.10%	0.41%	0.06%
Mean	9.2% ± 2.8	2.7% ± 0.5	82.7% ± 1.0	2.2% ± 0.8	0.33% ± 0.006	0.117% ± 0.03	0.587% ± 0.20	0.14% ± 0.07
Mfn2 f/f Vav-Cre
	9.42%	2.56%	83.00%	1.86%	0.03%	0.13%	0.54%	0.20%
	9.25%	2.55%	84.50%	2.09%	0.06%	0.13%	0.81%	0.20%
	11.30%	3.44%	80%	2.74%	0.03%	0.09%	0.57%	0.21%
Mean	10.0% ± 1.1	2.8% ± 0.5	82.5% ± 2.3	2.2% ± 0.5	0.040% ± 0.02	0.117% ± 0.02	0.64% ± 0.15	0.203% ± 0.01