Figure 1.

Generation and characterization of the Mdm2^PND/PND mice. (a) Schematic representation of the Mdm2^PND allele. Yellow ovals and broken line depict p53 response element mutations and a 184- bp deletion, respectively. (b) RT–qPCR for p53 targets in thymi of un-irradiated and irradiated Mdm2^PND/PND mice. n = 3,±s.e.m., *P < 0.05, **P < 0.01, ***P < 0.001. ns, non significant. (c) RT–qPCR for p53 targets in spleens of Mdm2^PND/PND mice. n = 3, ±s.e.m., *P < 0.05. (d) Western blot from testis lysate of wild-type and Mdm2^PND/PND mice. −C and +C represent negative and positive controls, respectively. (e) Representative TUNEL staining in thymus and testis of wild-type and Mdm2^PND/PND mice.