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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Free Radic Biol Med. 2016 Aug 8;99:225–233. doi: 10.1016/j.freeradbiomed.2016.08.005

Fig. 1.

Fig. 1

NQO1 binds SERPINA1 mRNA. (A) Schematic of the RIP assay using cytoplasmic HepG2 cell lysates under conditions that preserved mRNA-RBP associations. (B) Western blot analysis of NQO1 recovered in IP samples. (C) Data represent the Z-ratio and signal intensities of mRNAs in NQO1-IP relative to IgG-IP under CTRL and β-lap treatment. (D) RT-qPCR analysis revealed that the main NQO1 target among those tested was SERPINA1 mRNA. β-lap treatment caused a rapid loss in the amount of SERPINA1 mRNA bound to NQO1. Data represent the mean of three independent experiments ± SEM.