Table 2.
Plasmids used in this study.
| Name | Description | References |
|---|---|---|
| Gateway destination vectors | ||
| pAG414GPD-ccdB | Centromeric TRP PGPD-ccdB-TCYC1 | (Alberti et al., 2007) |
| pAG416ADH1-ccdB | Centromeric URA PADH1-ccdB-TCYC1 | This study |
| pAG416GPD-ccdB | Centromeric URA PGPD-ccdB-TCYC1 | (Alberti et al., 2007) |
| pAG416HXT7-ccdB | Centromeric URA PHXT7-ccdB-TCYC1 | This study |
| pAG423GPD-ccdB | 2μ HIS PGPD-ccdB-TCYC1 | (Alberti et al., 2007) |
| Holding vectors | ||
| pCS2656 | PGPD-T6ODM-TADH1 | (Thodey et al., 2014) |
| pCS2657 | PTEF1-CODM-TCYC1 | (Thodey et al., 2014) |
| pCS2661 | PTPI1-COR1.3-TSTE2 | (Thodey et al., 2014) |
| pCS3428 | PTEF1-yXMT1-TCYC1 | This study |
| pCS3429 | PTEF1-CaXMT1-TCYC1 | This study |
| pCS3430 | PGPD-PA0065-TADH1 | This study |
| pCS3431 | PTPI1-XPT1-TSTE2 | This study |
| Gateway product vectors | ||
| pCS3523 | Centromeric TRP PGPD-CaXMT1-TCYC1 | This study |
| pCS3524 | 2μ HIS PGPD-CaXMT1-TCYC1 | This study |
| pCS3525 | Centromeric TRP PGPD-CaMXMT1-TCYC1 | This study |
| pCS3526 | Centromeric URA PGPD-CaMXMT1-TCYC1 | This study |
| pCS3527 | Centromeric URA PGPD-CaMXMT2-TCYC1 | This study |
| pCS3528 | Centromeric TRP PGPD-CaDXMT1-TCYC1 | This study |
| pCS3529 | Centromeric URA PGPD-CaDXMT1-TCYC1 | This study |
| pCS3530 | Centromeric URA PGPD-CaCCS1-TCYC1 | This study |
| pCS3531 | Centromeric URA PGPD-yCCS1-TCYC1 | This study |
| pCS3532 | Centromeric URA PHXT7-yCCS1-TCYC1 | This study |
| pCS3533 | Centromeric URA PADH1-yCCS1-TCYC1 | This study |
| pCS3618 | Centromeric HIS PGPD-SAM2-TCYC1 | This study |
| Control vectors | ||
| pCS1 | Centromeric HIS empty control | This study |