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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Acta Neuropathol. 2016 Oct 4;132(6):827–840. doi: 10.1007/s00401-016-1623-4

Fig. 1.

Fig. 1

In vitro fibrillized recombinant WT SOD1 induces MND in G85R-SOD1:YFP injected mice. (a) Thioflavin T fluorescence of recombinant WT SOD1 while shaking at 37°C. n=2; mean ± SEM. (b) Representative image from EM analysis of WT SOD1 fibrils (n = 3). (c) Representative image from filter trap assay revealing HMW species formed in recombinant WT SOD1 samples (n = 3). (d) Kaplan-Meier survival curves show MND disease induction in G85R-SOD1:YFP mice injected within the spinal cord at P0 with WT SOD1 fibrils and one animal that developed disease following a control injection. (e) Representative image of inclusion pathology induced in the spinal cord of a diseased G85R-SOD1:YFP mouse that had been injected with WT SOD1 fibrils (n=5 mice, 16 sections per mouse).