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. Author manuscript; available in PMC: 2017 Oct 21.
Published in final edited form as: ACS Chem Biol. 2016 Aug 17;11(10):2752–2762. doi: 10.1021/acschembio.6b00404

Figure 2. Binding of peptides at PTHR1.

Figure 2

Binding to PTHR1 was assessed using cell membrane preparations derived from a HEK-293 cell line stably transfected to express human PTHR1. Experiments were carried out in the presence of GTPγS (10−5 M) using 125I-PTH(1–34) as a tracer radioligand. Binding values are reported in Table 1. Data are presented as means (± SEM) of three experiments, with each condition assessed in duplicate in each experiment. Curves represent fitting of a sigmoidal dose response with variable slope model to the data.