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. Author manuscript; available in PMC: 2016 Nov 13.
Published in final edited form as: Allergy. 2016 Mar 2;71(7):977–988. doi: 10.1111/all.12861

Figure 6.

Figure 6

ILC2s are a major source of serum IL-5 in mice exposed to Alternaria. Naïve IL-5venus cytokine receptor mice (BALB/c background) were exposed once i.n. to PBS or 50 µg Alternaria. (A) Lung single-cell suspensions were stained with a lineage cocktail Ab and analyzed for the IL-5venus signal by FACS. Right panel depicts CD25 and CD44 expression by the lineage-negative and IL-5venus-positive cell population in the Alternaria-exposed mice. (B) Lung single-cell suspensions were stained with a lineage cocktail Ab and Abs for CD25 and CD44, and expression of IL-5venus in the ILC2 population (Lin−CD25+CD44hi) was analyzed by FACS. Data are presented as representative of 2–4 mice in each group. (C and D) Wild-type C57BL/6 or Rag1−/− mice (C57BL/6 background) (C) or wild-type C57BL/6 mice or Il7ra−/− mice (C57BL/6 background) (D) were exposed i.n. to PBS or 50 µg Alternaria. Sera were collected 6 h later, and IL-5 levels were determined by ELISA. Data are shown as mean ± SEM (n = 4). **P < 0.01, between the groups indicated by horizontal lines.