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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Amino Acids. 2016 Sep 10;48(12):2831–2842. doi: 10.1007/s00726-016-2318-9

Fig. 4.

Fig. 4

Effect of knockdown of ATF4 in GCN2(+/+), GCN2(−/−), eIF2α(S/S) and eIF2α(A/A) MEFs on expression of ATF4, 4EBP1, ASNS and 4EBP2. Nonsense (NS, control) or ATF4 siRNA was transfected into GCN2(+/+), GCN2(−/−), eIF2α(S/S) and eIF2α(A/A) MEFs. After siRNA transfection, MEFs were cultured in sufficient medium (Suff) or in medium deficient in histidine (His-) or methionine (Met-) for 12h. ATF4, 4EBP1, ASNS and 4EBP2 mRNA levels after 12 h of culture in deficient medium after transfection of NS or ATF4 siRNA, expressed as fold the mean value for cells transfected with NS siRNA and cultured in sufficient medium, are shown for a) GCN2(+/+), b) GCN2(−/−), c) eIF2α(S/S) and d) eIF2α(A/A) MEFs. Values are means ± SEM for 3 separate experiments, with each experiment having 3 replicates. Bars not denoted by the same letter are significantly different by Student’s t-test comparison at p≤ 0.05.