Fig. 5. Oxidative stress is involved in RARα deletion-induced calcium mishandling.

Neonatal mouse cardiomyocytes were transfected with AdGFP or AdCre, and then treated with or without N-acetyl-cysteine (NAC, 5 mmol/L) for 2 and 24 h, protein expression and phosphorylation of Akt, CaMKIIδ, PLB, SERCA2a, RARα and α-tubulin determined by Western blot (A) and quantified (B). *p<0.05 vs AdGFP; †p<0.05 vs AdCre.