Figure 5. Chondrogenic differentiation of HDPCs isolated by three different methods. Alcian Blue staining of HDPCs-OG (a - d), HDPCs-ED (e - h) and HDPCs-Combined (i - l) at 14 and 28 days after the induction of differentiation. Scale bar: 50 µm. HDPCs, human dental pulp cells; HDPCs-OG, HDPCs were isolated by the outgrowth method; HDPCs-ED, HDPCs were isolated by the enzymatic digestion method (collagenase/dispase/trypsin); HDPCs-Combined, HDPCs were isolated by the combination of both methods.
