Figure 6.

Cells were treated with local anesthetics at the concentrations of ED50 for 24 h and then total proteins were detected by western blot using beta-actin as an internal control. (A) Local anesthetics downregulated the expression level of cyclin D1 compared with NC groups; (B) local anesthetics affected the protein levels of the intrinsic mitochondrial pathway; (C) local anesthetics affected the protein levels of the extrinsic death receptor pathway; (D) local anesthetics activated MAPK pathways.