KIFC1 expression could be inhibited by KIFC1 siRNA. (A-C) The expression of KIFC1 mRNA level was measured following the treatment of A549, H1299 and SPC-A1 cells with siRNA by qRT-PCR; (D-F) Western blot analyze KIFC1 expression after the treatment of A549, H1299 and SPC-A1 cells with siRNA in protein level. The letters (A,D), (B,E) and (C,F) represent A549, H1299 and SPC-A1 cell lines, respectively. *, P<0.05; **, P<0.01; ***, P<0.001. KIFC1, kinesin family member C1; qRT-PCR, quantitative reverse-transcription polymerase chain reaction.