Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Nov 14;7:1683. doi: 10.3389/fpls.2016.01683

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016 Lowder, Malzahn and Qi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Delivery of CRISPR reagents to plant cells and tissues. (A) Floral dip transformation of Arabidopsis with transgenic T-DNA carrying Agrobacteria. (B) Transient inoculation of plant leaf tissue or calli with Agrobacteria harboring Cas9 and gRNA T-DNA. (C) Viral vector delivery causes a transiently transformed plant (at left) to develop systemic infection upon viral capsid replication after initial transformation of vector DNA. (D) Transient particle bombardment of plant leaf tissue using a gene gun with Cas9 and gRNA or (E) gRNAs only to stable Cas9-expressing transgenic plants. (F) Ribonucleoprotein (RNP) complex delivery directly to protoplasts using PEG transformation or (G) RNA delivery directly to protoplasts (shown here) using PEG transformation or calli using “gene gun” as in (D).