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. 2016 Nov 7;39(3):316–328. doi: 10.1016/j.devcel.2016.10.009

Figure 5.

Figure 5

EphB3b Controls LPM Polarity and Asymmetric Movement

(A–E′) EphrinB1 and EphB3b regulate LPM polarity in the foregut region. α-ZO-1 staining in MO-ephb3b embryos reveals that junctions in the LPM form similar to controls at 22 hpf (A–B′); while at 32 hpf, ZO-1 is mislocalized (arrowheads) in MO-ephrinb1 and MO-ephb3b embryos (C–E′). Yellow arrowheads specify the midline and reveal impaired gut looping in MO-ephrinb1 and MO-ephb3b (C–E).

(F–G′) casanova mutants exhibit ZO-1 localization defects in the LPM (arrowheads) at 32 hpf.

(A–G′) Transverse sections at liver level, left side to the right; dashed lines delineate the endoderm (green) and LPM (yellow); (A′–G′) are magnifications of the areas indicated by a box in (A–G).