Table 1. Intra- and inter-assay CVs of the duplex real-time PCR with the internal standard, single real-time PCR with the external standard, and PicoGreen assay.
| Assays | Inter-assay CVs (n = 20) | Inter-assay CVs (n = 20) | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 1,000 ng/mL | 100 ng/mL | 10 ng/mL | 1.0 ng/mL | 0.1 ng/mL | 1,000 ng/mL | 100 ng/mL | 10 ng/mL | 1.0 ng/mL | 0.1 ng/mL | |
| Internal Standard | 2.63% | 2.96% | 4.43% | 15.21% | 53.96% | 2.49% | 3.15% | 5.39% | 14.17% | 76.16% |
| External Standard | 4.90% | 8.84% | 16.29% | 60.50% | 128.47% | 5.86% | 10.19% | 17.33% | 65.68% | 144.71% |
| PicoGreen | 3.99% | 5.81% | 24.49% | 76.10% | - | 5.55% | 6.22% | 30.21% | 81.62% | - |